study of comparative effects of harmine and ghrelin agonist on insulin and c-peptide secretion

Aim and Background: The ability to secrete insulin from the islets of Langerhans on the one hand and suppress the factors that increase serum glucose concentrations on the other; It is very important in patients with metabolic disorders such as diabetes. The aim of this study was to compare the effects of harmine with ghrelin agonist (as a suppressor of insulin secretion) on insulin secretion and c-peptide in PANC-1 cell line and intracellular glucose concentration in HT1080 cell line.  Materials and Methods: To do this, cell lines were purchased from the Iranian Genetic Resources Center and after cell passage and preparation, were treated with doses of 0/01, 0/1, 1, 10 and 100 μg /ml harmin and ghrelin agonists with doses of 0/05, 0/5, 5, 50 and 500 μg / ml. While performing MTT test, insulin secretion and c-peptide were measured by ELISA. Results: The results showed that harmine dose-dependently increased insulin and c-peptide secretion as well as increased intracellular glucose and had the opposite effect of ghrelin agonist.  Conclusion: Harmine analogues could be a unique therapeutic promise for human diabetes and possibly serve as inhibitors of ghrelin agonists.